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KMID : 0361020000430060631
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Korean Journal of Otolaryngology - Head and Neck Surgery
2000 Volume.43 No. 6 p.631 ~ p.637
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Expression of p53, PCNA, EGFR in Rat Submandibular Gland Tumor Induced Experimentally
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1유영삼/1Young Sam Yoo
1우훈영/1이창환/1최정환/2서민/1Hoon Young Woo/1Chang Hwan Lee/1Jung Hwan Choi/2Min Seo
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Abstract
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-Abstract-
Background and Objectives : Animal models of cancer are frequently use in many studies. In this study,
we tried to make a model of SMG cancer using rats. We used SMG tissues from rats for the analysis of
expression of p53, PCNA and EGFR. Materials and Methods : Pellets of 4 6 mg DMBA were implanted
into the submandibular glands of 47 female Sprague-Dawley rats which were sacrificed at 4, 8, 10, 14,
and 18 weeks after DMBA implantation. Thirteen rats were sacrificed at the beginning of experiment and
were used as a control group. Histopathological and immunohistochemical staining for p53, PCNA and
EGFR were carried out suing tissues from rat submandibular glands (SMGs). Results : Histopathological
features during carcinogenesis were as follows ; 1)Well differentiated squamous cell carcinomas (SCCs)
were first developed at the 8th week of the experiment. 2) In 2 out of 12 cases, fibrosarcoma and SCC
were induced simultaneously at the 18th week of experiment. P53-positive unclei were not observed in
this experiment. PCNA-positive unclei were mainly confined to the basal cells of the morphologically
altered ducts at the 4th week of experiment. Squamous metaplastic cells and well differentiated SCC s
showed strong reactivity to PCNA. The PCNA index was about 40% in the tumour incduced group.
PCNA indices were 28.8%, 48.1%, 44.6% and 37.8% at the 4th, 10th, 14th and 18th week of the
experiment, respectively. In the control group, PCNA index was 6.1%. Increase of PCNA index in the
experimental groups was statistically significant compared with control group. The staining intensity for
EGFR increased along with the duration of exposure to DMBA. Conclusion : SCC model of rat SMGs
could be developed successfully by implantation of DMBA. In this model, p53 mutation does not seem to
play any important role during carcinogenesis. PCNA and EGFR seem to be closely related to
carcinogenesis of SMGs in this model.
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KEYWORD
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